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Miniaturized asymmetrical flow field‐flow fractionation: Application to biological vesicles
Author(s) -
Oh Sunok,
Kang Dukjin,
Ahn SungMin,
Simpson Richard J.,
Lee BongHee,
Moon Myeong Hee
Publication year - 2007
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200600394
Subject(s) - field flow fractionation , fractionation , exosome , microvesicles , chromatography , polystyrene , chemistry , flow (mathematics) , channel (broadcasting) , vesicle , nanotechnology , micrometer , materials science , analytical chemistry (journal) , polymer , computer science , biochemistry , optics , membrane , microrna , geometry , mathematics , physics , organic chemistry , gene , computer network
Asymmetrical flow field‐flow fractionation (AFlFFF) has been carried out in a miniaturized channel by reducing the channel dimensions. Performance of the miniaturized AFlFFF (mAFlFFF) channel was evaluated with standard proteins and polystyrene latex spheres from nanometer to micrometer size. By reducing the channel dimension, proteins or particulate materials can be separated within a few minutes without a significant loss in resolution. The mAFlFFF channel was applied for the separation of exosomes harvested from immortalized human mesenchymal stem cell line. It shows a potential to fractionate exosome vesicles according to sizes which can be useful for proteomic studies in relation to immunotherapeutic applications.