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Temperature effects on separation on zirconia columns: Applications to one‐ and two‐dimensional LC separations of phenolic antioxidants
Author(s) -
Cacciola Francesco,
Jandera Pavel,
Mondello Luigi
Publication year - 2007
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200600387
Subject(s) - cubic zirconia , chemistry , carbon fibers , chromatography , selectivity , analytical chemistry (journal) , phase (matter) , thermal stability , atmospheric temperature range , wine , materials science , organic chemistry , ceramic , thermodynamics , physics , food science , composite number , composite material , catalysis
The effects of temperature and mobile phase on LC chromatographic separation of phenolic antioxidants on zirconia‐based columns were investigated. Unlike silica‐based materials, zirconia columns show excellent thermal stability over a wide range of temperatures and enable high‐temperature separations. Enthalpic and entropic contributions to the retention of phenolic compounds on ZR‐Carbon and ZR‐Carbon C18 columns were determined from retention versus temperature plots in order to elucidate the retention mechanism of sample compounds over the temperature range up to 140°C. High‐temperature liquid chromatography on ZR‐Carbon columns was used for comprehensive LC×LC two‐dimensional separation systems based on the different selectivity of a Zorbax SB micro‐column used in the first dimension and a ZR‐Carbon column used in the second dimension. Two‐dimensional LC×LC systems employing a setup with two alternately operated parallel ZR‐Carbon columns in the second dimension were used for the analysis of phenolic antioxidants in beer and wine samples.