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Comparison of comprehensive two‐dimensional gas chromatography‐time‐of‐flight mass spectrometry and gas chromatography‐mass spectrometry for the qualitative characterisation of roasted barley by solid‐phase microextraction
Author(s) -
Bianchi Federica,
Careri Maria,
Conti Chiara,
Musci Marilena,
Vreuls Renè
Publication year - 2007
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200600380
Subject(s) - chromatography , solid phase microextraction , mass spectrometry , chemistry , gas chromatography–mass spectrometry , gas chromatography , extraction (chemistry) , time of flight mass spectrometry , two dimensional chromatography , volatile organic compound , ion , organic chemistry , ionization
Volatile compounds of roasted barley used in the production of barley coffee, the most common coffee substitute, were analysed by using solid‐phase microextraction (SPME) followed by GC‐MS and comprehensive GC×GC‐TOF‐MS, respectively. The optimised SPME extraction conditions in terms of selection of the fibre coating, extraction time and extraction temperature allowed to obtain the highest GC response, thus enhancing the identification capabilities of the developed method. As for the SPME‐GC×GC‐TOF‐MS analysis, 64 compounds with similarity, reverse and probability values above 800, 900 and 6000, respectively, were identified, by using a polar×apolar column set combination; in contrast, GC‐MS was able to identify a lower number of compounds, i. e. 40 volatiles.