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Simultaneous qualification and quantification of eight triterpenoids in Radix Achyranthis Bidentatae by high‐performance liquid chromatography with evaporative light scattering detection and mass spectrometric detection
Author(s) -
Li Juan,
Li Ping,
Li HuiJun,
Song Yue,
Bi ZhiMing,
Li YanJing
Publication year - 2007
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200600341
Subject(s) - chromatography , repeatability , chemistry , chromatography detector , formic acid , high performance liquid chromatography , analyte , detection limit , analytical chemistry (journal) , radix (gastropod) , mass spectrometry , calibration curve , botany , biology
An HPLC with evaporative light scattering detection (ELSD) and ESI‐MS was established for the simultaneous determination of eight triterpenoids in Radix Achyranthis Bidentatae. The optimal chromatographic conditions were achieved on a Zorbax C18 column by linear gradient elution with 0.08% v/v aqueous formic acid and ACN as the mobile phase at the flow rate of 0.8 mL/min. Temperature for the detector drift tube was set at 101°C and the nitrogen flow rate was 2.8 L/min. The identities of the analytes were accomplished by comparing retention times and mass data with those of reference compounds. The validation of the method included tests of linearity, sensitivity, repeatability, recovery, and stability. All the calibration curves of the eight triterpenoids showed good linear regression ( R 2 >0.997) within the test ranges. The method provides desirable repeatability with overall intra‐ and interday variations of less than 4.9%. The obtained recoveries varied between 93.6 and 98.1% while the RSDs were below 3.9% ( n = 3). The analysis results indicate that the content of investigated triterpenoids in Radix Achyranthis Bidentatae from different locations was greatly diverse, and the triterpenoids could be used as chemical markers for the discrimination of genuine and ungenuine crude drugs.