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Elevated temperature–extended column length conventional liquid chromatography to increase peak capacity for the analysis of tryptic digests
Author(s) -
Sandra Pat,
Vanhoenacker Gerd
Publication year - 2007
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200600329
Subject(s) - chromatography , chemistry , analytical chemistry (journal) , high performance liquid chromatography , column (typography) , column chromatography , monolithic hplc column , structural engineering , connection (principal bundle) , engineering
High efficiency separations (200 000 plates) were obtained on conventional LC equipment by coupling 8×25 cm×2.1 (or 4.6) mm id×5 μm d p ODS columns (total length 2 m) and operation at 60°C using a dedicated LC oven. The peak capacity in this 1‐D set‐up was 900 for the separation of human serum tryptic peptides analyzed after depletion of six highly abundant proteins. The chromatographic performance of an elevated temperature–extended column length conventional LC is highlighted.