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Determination of polyacetylenes in carrot roots ( Daucus carota L.) by high‐performance liquid chromatography coupled with diode array detection
Author(s) -
Christensen Lars P.,
Kreutzmann Stine
Publication year - 2007
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200600325
Subject(s) - daucus carota , chromatography , chemistry , calibration curve , elution , acetonitrile , high performance liquid chromatography , analytical chemistry (journal) , detection limit , botany , biology
A new high‐performance liquid chromatographic method with diode array detection was developed for the separation and simultaneous determination of the carrot polyacetylenes falcarindiol (FaDOH), falcarindiol 3‐acetate (FaDOAc) and falcarinol (FaOH) in carrot root extracts. The optimal chromatographic conditions were achieved on a C18 column with a linear gradient elution of water and acetonitrile as mobile phases, at the flow rate of 1.0 mL/min. All calibration curves of the three carrot polyacetylenes showed good linear regression ( R 2 >0.998) within the test ranges. The developed method showed good precision for quantification of all polyacetylenes with overall intraday and interday variation of less than 3.3% and with average recovery rates of 99.2, 96.8 and 99.7% for FaDOH, FaDOAc and FaOH, respectively. The LOD (S/N = 3) and LOQ (S/N = 10) were less than 0.19 and 0.42 μg/mL, respectively, for all analytes. The established method was successfully used to determine the spatial distribution of FaDOH, FaDOAc and FaOH in six carrot genotypes (Bolero, Independent, Line 1, Mello Yello, Purple Haze and Tornado) by analysing peeled carrots and the corresponding peels for these polyacetylenes.