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Identification of neuropeptides in rat brain rhinencephalon
Author(s) -
Mihailova Albena,
Karaszewski Bartosz,
Hauser Roman,
Lundanes Elsa,
Greibrokk Tyge
Publication year - 2007
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200600317
Subject(s) - chemistry , neuropeptide , chromatography , mass spectrometry , peptide , solid phase extraction , high performance liquid chromatography , extraction (chemistry) , biochemistry , receptor
A capillary two‐dimensional liquid chromatography method coupled with ion trap mass spectrometry has been used for separation and identification of neuropeptides in rat rhinencephalon. Animals of three different age groups were exposed to slow and quick CO 2 influx. The neuropeptides were extracted by solid phase extraction and the purified extracts were analysed by 2‐D HPLC. The compounds were fractionated (strong cation exchange column), trapped and separated, and MS/MS fragment mass spectra were used for identification. About thirty peptide compounds were identified. A significant difference between concentration levels of “stressed” (quick CO 2 influx) and “non‐stressed” (slow CO 2 influx) rats was found for 25 of the identified peptides.