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Comparison of various extraction techniques for isolation and determination of isoflavonoids in plants
Author(s) -
Bajer Tomáš,
Adam Martin,
Galla Lubomír,
Ventura Karel
Publication year - 2007
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200600306
Subject(s) - chromatography , extraction (chemistry) , isolation (microbiology) , chemistry , biology , bioinformatics
In the present paper, the following extraction techniques have been used for extracting isoflavonoids from the species Matricaria recutita, Rosmarinus officinalis, Foeniculum vulgare , and Agrimonia eupatoria L.: supercritical fluid extraction (SFE), pressurized fluid extraction, matrix solid phase dispersion, ultrasonic extraction in an ultrasonic bath (USE) and by means of an ultrasonic homogeniser (HOM), extraction by means of Soxhlet apparatus (SOX), and solid phase extraction. Experimental optimization of all techniques has been carried out using a soybean flour. Subsequent analyses of the extracts were carried out by liquid chromatography with UV detection. The maximum yields of daidzein and genistein were obtained by extraction with the SOX, USE, and HOM techniques. The maximum yields of apigenin and biochanin A from herb samples were obtained by SFE.