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Rapid determination of polyprenylated xanthones in gamboge resin of Garcinia hanburyi by HPLC
Author(s) -
Song JingZheng,
Yip YueKeung,
Han QuanBin,
Qiao ChunFeng,
Xu HongXi
Publication year - 2007
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200600294
Subject(s) - chemistry , chromatography , epimer , high performance liquid chromatography , garcinia , elution , methanol , bromide , gambogic acid , organic chemistry , biochemistry , in vitro , medicine , traditional medicine
A rapid ion‐pair HPLC method was developed and validated for the determination of eight polyprenylated xanthones including three pairs of epimers, namely morellic acid (MA), 30‐hydroxygambogic acid (HGA), 30‐hydroxyepigambogic acid (HEGA), isogambogic acid (IGA), epiisogambogic acid (EIGA), gambogenic acid (GNA), gambogic acid (GA), and epigambogic acid (EGA), in gamboge resin of Garcinia hanburyi . The separation was performed on a narrow bore C 8 column with isocratic elution using a mixture of methanol‐ACN‐40 mM KH 2 PO 4 buffer (37.5 : 37.5 : 25 v/v/v, containing 0.1% tetradecyltrimethylammonium bromide). The newly developed method was used to determine the contents of the eight compounds present in the gamboge. Results showed that GA and EGA are the dominant components of gamboge. The content ratio of each epimer pair remained constant, indicating that the content ratio of epimers can be used as a specific characteristic for the quality control of gamboge.