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Determination of the main bioactive metabolites of Radix Salvia miltiorrhizae in Compound Danshen Dripping Pills and the tissue distribution of Danshensu in rabbit by SPE‐HPLC‐MS n
Author(s) -
Xiaohui Zheng,
Xinfeng Zhao,
Xin Zhao,
Shixiang Wang,
Yinmao Wei,
Jianbin Zheng
Publication year - 2007
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200600287
Subject(s) - chemistry , radix (gastropod) , chromatography , high performance liquid chromatography , salvia miltiorrhiza , salvia , solid phase extraction , rosmarinic acid , extraction (chemistry) , isopropyl , biochemistry , traditional medicine , medicine , botany , organic chemistry , biology , antioxidant , alternative medicine , traditional chinese medicine , pathology
A fast solid phase extraction‐high performance liquid chromatography‐ion trap mass spectrometry (SPE‐HPLC‐MS n ) method is presented for quantitative determination of 3‐(3,4‐dihydroxyphenyl)‐2‐hydroxylpropionic acid (Danshensu, DSS) from Compound Danshen Dripping Pills (CDDP) in rabbits' heart, liver, kidney, and lung and for qualitative analysis of the bioactive metabolites of Radix Salvia miltiorrhizae (DS) from CDDP in these tissues. The proposed method was validated with a detection limit of 0.06 ng/mL and a linear range of 5.0–200 ng/mL for DSS. The main metabolites of DS were pyrocatechol, vanillic acid, isopropyl 3‐(3,4‐dihydroxyphenyl)‐2‐hydroxypropanoate, and 2‐hydroxyl‐3‐(3,4‐dihydroxyphenyl)propinonic sulfuric anhydride. In vitro pharmacological experiments demonstrated that isopropyl 3‐(3,4‐dihydroxyphenyl)‐2‐hydroxypropanoate had a distinct vasodilatating action.