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Identification and determination of glycosides in tobacco leaves by liquid chromatography with atmospheric pressure chemical ionization tandem mass spectrometry
Author(s) -
Pang Tao,
Yuan Zhongyi,
Dai Yongsheng,
Wang Chang,
Yang Jun,
Peng Liming,
Xu Guowang
Publication year - 2007
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200600236
Subject(s) - atmospheric pressure chemical ionization , chemistry , chromatography , rutin , mass spectrometry , glycoside , high performance liquid chromatography , kaempferol , tandem mass spectrometry , chemical ionization , analytical chemistry (journal) , quercetin , ionization , ion , organic chemistry , antioxidant
HPLC‐atmospheric pressure chemical ionization MS (HPLC‐APCI‐MS) was used to screen and identify glycosides in tobacco leaf. MS/MS and MS 3 and photodiode array (PDA) detection were also used in the characterization. A total of 12 glycosides were found and four of them were identified based on their abundant [M + H] + ions, UV spectra, and MS/MS analysis and they are scopolin, rutin, quercetin‐3‐glycoside, and kaempferol‐3‐rutinoside. Analytical characteristics of the method were investigated. The contents of these glycosides were obtained and compared based on the relative peak area to the internal standard in seven kinds of tobacco leaf.