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Coupled‐column liquid chromatography combined with postcolumn photochemical derivatization and fluorescence detection for the determination of herbicides in groundwater
Author(s) -
Mughari Ahmed R.,
Martínez Galera María,
Parrilla Vázquez Piedad,
Santiago Valverde Rosario
Publication year - 2007
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200600234
Subject(s) - chromatography , chemistry , derivatization , analyte , propanil , detection limit , fluorescence spectroscopy , high performance liquid chromatography , fluorescence , analytical chemistry (journal) , pesticide , physics , quantum mechanics , agronomy , biology
This study examines the application of coupled‐column LC‐photochemically induced fluorimetry‐fluorescence detection (LC‐LC‐PIF‐FD), demonstrating its potential for the quantitative and selective detection of six herbicides, including propanil and the phenylureas monuron, monolinuron, chlorotoluron, diuron and neburon in groundwater samples. An AQUASIL C 18 50×4.6 mm 2 id column coupled to an AQUASIL C 18 150×4.6 mm 2 id column for analyte clean‐up and determination were used, respectively. A simple SPE with C 18 cartridges was carried out, yielding average recoveries between 80 and 112% ( n = 6) with RSDs between 0.5 and 9%. The LODs ranged from 0.0083 to 0.0833 μg/L in the groundwater samples.