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Simultaneous determination of catecholamines and amino acids by micellar electrokinetic chromatography with laser‐induced fluorescence detection
Author(s) -
Zhou Lei,
Wang Shumin,
Tian Kan,
Dong Yuming,
Hu Zhide
Publication year - 2007
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200600219
Subject(s) - chemistry , micellar electrokinetic chromatography , derivatization , chromatography , amino acid , fluorescence , laser induced fluorescence , fluorescence spectroscopy , quantitative analysis (chemistry) , high performance liquid chromatography , detection limit , biochemistry , physics , quantum mechanics
A selective and sensitive method was developed for separation and simultaneous determination of catecholamines and amino acids by MEKC with LIF. Interestingly enough, such work has been firstly performed on catecholamines derivatized with 4‐chloro‐7‐nitro‐2,1,3‐benzoxadiazole and the detailed derivatization mechanism was discussed. After derivatization at 60°C for 20 min, NBD‐labeled catecholamines and amino acids were separated in a buffer system containing 10 mM sodium tetraborate‐Na 2 HPO 4 , 20 mM SDS, and 10% v/v ACN at pH 9.75. SDS micelles were employed to improve the fluorescence intensity of catecholamine derivatives efficiently. Under optimum conditions, two catecholamines and 11 amino acids were separated in a short 13 min analysis time and the RSDs for migration time and peak area were less than 0.60 and 6.50%, respectively. The method was successfully applied for the quantification of catecholamines and amino acids in Portulaca oleracea L., human urine sample, and mixed injection sample.