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On‐line hyphenation of supercritical fluid extraction and two‐dimensional high performance liquid chromatography‐atmospheric pressure chemical ionization tandem mass spectrometer for the analysis of Ganoderma lucidum
Author(s) -
Zhang Jie,
Zhang Lihua,
Duan Jicheng,
Liang Zhen,
Zhang Weibing,
Huo Yushu,
Zhang Yukui
Publication year - 2006
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200600217
Subject(s) - chromatography , atmospheric pressure chemical ionization , chemistry , mass spectrometry , supercritical fluid chromatography , tandem , supercritical fluid , analytical chemistry (journal) , extraction (chemistry) , supercritical fluid extraction , atmospheric pressure , chemical ionization , tandem mass spectrometry , high performance liquid chromatography , ionization , liquid chromatography–mass spectrometry , materials science , ion , organic chemistry , composite material , oceanography , geology
A novel on‐line system combining supercritical fluid extraction (SFE) and two‐dimensional high performance liquid chromatography (2D‐HPLC) was developed. A trap column and two three‐port valves were employed to couple SFE and 2D‐HPLC system, which was composed of a CN column and a monolithic silica column, connected by a 10‐port dual‐position valve. The analytes extracted by supercritical CO 2 were completely transferred to the 2D‐HPLC system. After separation in two orthogonal modes, the eluents were delivered to APCI‐tandem‐MS for identification of the samples. In this way, sample preparation, separation, detection, and identification were integrated into an on‐line system permitting analysis of the fruiting bodies of Ganoderma lucidum , and at least 73 components in the extract were resolved with calculated peak capacity of up to 1643.