Premium
Simple determination of betamethasone and chloramphenicol in a pharmaceutical preparation using a short monolithic column coupled to a sequential injection system
Author(s) -
Šatínský Dalibor,
Chocholouš Petr,
Salabová Markéta,
Solich Petr
Publication year - 2006
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200600204
Subject(s) - betamethasone , chromatography , simple (philosophy) , monolithic hplc column , chloramphenicol , column (typography) , chemistry , high performance liquid chromatography , computer science , medicine , antibiotics , telecommunications , philosophy , biochemistry , epistemology , frame (networking) , immunology
This contribution describes the use of a new separation method based on a reversed‐phase sequential injection chromatography (SIC) technique for simultaneous determination of chloramphenicol and betamethasone in pharmaceutical eye drops. A short monolithic column coupled with a sequential injection analysis (SIA) system enabled separation of two compounds in one step. A Chromolith Flash RP‐18e, 25×4.6 mm column with a 5 mm precolumn (Merck, Germany) and a FIAlab 3000 system (USA) with a 6‐port selection valve and 5 mL syringe were used for sequential injection chromatographic separations in this study. The mobile phase used was acetonitrile‐water (30 : 80, v/v), flow rate 0.48 mL/min; UV detection was at two wavelengths, i. e. , 241 and 278 nm (absorption maxima of betamethasone and chloramphenicol, respectively). The basic validation parameters showed good results: linearity of determination for both compounds including internal standard (propylparaben) >0.999; repeatability of determination (RSD) in the range 0.8–1.7% at two different concentration levels, and detection limits in the range 0.5–1.0 mg/mL. The chromatographic resolution between compound peaks was greater than 2.1 and the analysis time was less than 8 min under optimal conditions. The developed sequential injection chromatography method was compared with the HPLC method and was found to be applicable for routine analysis of active compounds in pharmaceutical preparations.