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Development and validation of an RP‐HPLC method for quantitative determination of vanillin and related phenolic compounds in Vanilla planifolia
Author(s) -
Sinha Arun Kumar,
Verma Subash Chandra,
Sharma Upendra Kumar
Publication year - 2007
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200600193
Subject(s) - vanillin , vanillic acid , chemistry , chromatography , ferulic acid , acetic acid , high performance liquid chromatography , alcohol , phenolic acid , methanol , organic chemistry , antioxidant
A simple and fast method was developed using RP‐HPLC for separation and quantitative determination of vanillin and related phenolic compounds in ethanolic extract of pods of Vanilla planifolia . Ten phenolic compounds, namely 4‐hydroxybenzyl alcohol, vanillyl alcohol, 3,4‐dihydroxybenzaldehyde, 4‐hydroxybenzoic acid, vanillic acid, 4‐hydroxybenzaldehyde, vanillin, p ‐coumaric acid, ferulic acid, and piperonal were quantitatively determined using ACN, methanol, and 0.2% acetic acid in water as a mobile phase with a gradient elution mode. The method showed good linearity, high precision, and good recovery of compounds of interest. The present method would be useful for analytical research and for routine analysis of vanilla extracts for their quality control.