Premium
Headspace solid‐phase microextraction of cannabinoids in human head hair samples
Author(s) -
Dizioli Rodrigues de Oliveira Carolina,
Yonamine Mauricio,
de Moraes Moreau Regina Lucia
Publication year - 2007
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200600192
Subject(s) - cannabidiol , cannabinol , chromatography , solid phase microextraction , extraction (chemistry) , chemistry , analyte , hair analysis , calibration curve , gas chromatography–mass spectrometry , detection limit , cannabis , mass spectrometry , medicine , alternative medicine , pathology , psychiatry
A fast method was optimized and validated with the aim to detect cannabinoids (cannabidiol, cannabinol, and Δ‐9‐tetrahydrocannabinol) in human head hair samples. The method was based on an initial procedure of external decontamination of hair samples (10 mg) with petroleum ether, followed by alkaline digestion and further extraction of cannabinoids by means of a headspace solid‐phase microextraction technique (HS‐SPME). GC‐MS was used to identify and quantify the analytes in SIM mode. The LOQs and LODs obtained were 0.07 and 0.12 ng/mg, respectively, for all the studied cannabinoids. The method proved to be simple, rapid, and precise. By using the weighted least squares linear regression (weighting factor 1/ x 2 ), the accuracy of the analytical method was improved at the lower end of the calibration curve (from 0.12 to 12 ng/mg; r >0.98). Hair samples collected from eight volunteers (in‐patients of a drug abuse rehabilitation clinic) were submitted to the proposed method. Detection of the drugs was observed in samples of the volunteers who reported frequent marijuana use (at least ten times a week).