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An evaluation of GC‐MS and HPLC‐FD methods for analysis of protein binders in paintings
Author(s) -
Přikryl Petr,
Havlíčková Lenka,
Pacáková Věra,
Hradilová Janka,
Štulík Karel,
Hofta Petr
Publication year - 2006
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200600171
Subject(s) - derivatization , chromatography , chemistry , chloroformate , reagent , hydrolysis , high performance liquid chromatography , organic chemistry
Two chromatographic methods have been compared for analysis of protein‐binding media used in paintings, namely, HPLC with fluorescence detection and GC‐MS. The proteins were hydrolyzed to the corresponding amino acids (AAs) by gaseous HCl and the AAs were derivatized with methyl chloroformate, followed by GC‐MS or by HPLC after derivatization with the AccQ fluorescence reagent. The hydrolysis, derivatization reactions and the chromatographic procedures have been optimized and applied to standard binding media, model and real samples of paintings. The methods have been compared and critically evaluated.