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Field‐flow fractionation of proteins, polysaccharides, synthetic polymers, and supramolecular assemblies
Author(s) -
Ratanathanawongs Williams S. Kim,
Lee Dean
Publication year - 2006
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200600151
Subject(s) - field flow fractionation , multiangle light scattering , macromolecule , branching (polymer chemistry) , fractionation , supramolecular chemistry , polymer , chemistry , copolymer , light scattering , nanotechnology , materials science , chromatography , scattering , molecule , organic chemistry , biochemistry , physics , optics
This review summarizes developments and applications of flow and thermal field‐flow fractionation (FFF) in the areas of macromolecules and supramolecular assemblies. In the past 10 years, the use of these FFF techniques has extended beyond determining diffusion coefficients, hydrodynamic diameters, and molecular weights of standards. Complex samples as diverse as polysaccharides, prion particles, and block copolymers have been characterized and processes such as aggregation, stability, and infectivity have been monitored. The open channel design used in FFF makes it a gentle separation technique for high‐ and ultrahigh‐molecular weight macromolecules, aggregates, and self‐assembled complexes. Coupling FFF with other techniques such as multiangle light scattering and MS provides additional invaluable information about conformation, branching, and identity.