Premium
Determination of amphetamines in human urine by liquid chromatography with fluorimetric detection using a solid‐phase extraction procedure
Author(s) -
Bugamelli Francesca,
Mandrioli Roberto,
Cavallini Annalisa,
Baccini Cesare,
Conti Matteo,
Raggi Maria Augusta
Publication year - 2006
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200600139
Subject(s) - chromatography , solid phase extraction , chemistry , analyte , mdma , urine , high performance liquid chromatography , extraction (chemistry) , ecstasy , triethylamine , bioanalysis , detection limit , medicine , biochemistry , organic chemistry , sociology , anthropology , pharmacology
A precise and feasible HPLC method has been developed for the analysis of amphetamine (AMPH), methamphetamine (MAMPH) and methylenedioxymethamphetamine (MDMA, ecstasy) in human urine. A chromatographic run on a C8 Genesis (150 mm×4.6 mm, 5 μm) column maintained at 30°C lasts about 17 min, using a mobile phase composed of ACN (12%) and a pH 2.5 phosphate buffer (88%) containing 0.3% triethylamine. Mirtazapine was used as the internal standard. Good linearity was found in the 100–2000 ng/mL concentration range for AMPH and MAMPH and in the 12–2000 ng/mL concentration range for MDMA. The pretreatment of urine samples was carried out by means of a careful SPE procedure on C2 cartridges. The extraction yields were very satisfactory for all analytes, with average values greater than 97%. The leading conditions allowed the determination of AMPH, MAMPH and MDMA with satisfactory precision and accuracy. The method has been successfully applied to the determination of the analytes in urine of AMPH users.