Premium
Separation and identification of the organic acids in Angelicae Radix and Ligustici Rhizoma by HPLC and CE
Author(s) -
Huang WenYing,
Sheu ShuennJyi
Publication year - 2006
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200600136
Subject(s) - chemistry , chromatography , high performance liquid chromatography , protocatechuic acid , ferulic acid , radix (gastropod) , caffeic acid , phthalic acid , vanillic acid , phenolic acid , organic chemistry , botany , biology , antioxidant
Angelicae Radix (AR) and Ligustici Rhizoma (LR) are both derived from the Umbelliferae plants and contain similar organic acids as their bioactive compounds. Nine of these organic acids, including nicotinic acid, protocatechuic acid, phthalic acid, folinic acid, p ‐hydroxybenzoic acid, folic acid, vanillic acid, caffeic acid, and ferulic acid were separated by HPLC and CE. Detection at 210 nm with a linear gradient containing 20 mM KH 2 PO 4 (pH 3.5) and H 2 O–CH 3 CN in HPLC and with a buffer solution containing 10 mM LTAC, 2 mM Na 2 HPO 4 , 9 mM Na 2 B 4 O 7 (pH 9.56), and CH 3 CN in CE were found to be the most efficient eluents for this separation. The contents of the nine components in crude extracts of either AR or LR could easily be determined within 60 min by LC and within 20 min by CE. The structures of the individual peaks in the LC chromatogram were identified by LC‐MS. The effects of buffers on the separation and validation of the two methods were examined.