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Simultaneous analysis of nine active components in Gegen Qinlian preparations by high‐performance liquid chromatography with diode array detection
Author(s) -
Chen Lihong,
Wang Qiang,
Liu Jining
Publication year - 2006
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200600123
Subject(s) - chromatography , baicalin , daidzin , puerarin , chemistry , high performance liquid chromatography , coptisine , palmatine , chromatography detector , baicalein , liquiritin , daidzein , pharmacology , medicine , alternative medicine , pathology , genistein
HPLC with diode array detection (HPLC/DAD) was employed to determine the quantities of puerarin, daidzin, daidzein, berberine, palmatine, coptisine, baicalin, baicalein, and glycyrrhizin in Gegen Qinlian preparations of three different pharmaceutical forms including decoction, dispensing granule and pill. The calibration curves for the nine bioactive components were linear in the given concentration ranges. The precision of the method was in the range of 0.2–5.0% (RSD), and the recoveries of this method were between 96.5 and 104.1%. The proposed method was applicable to analyze Gegen Qinlian preparations.