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Simultaneous determination of 11 saponins in Panax notoginseng using HPLC‐ELSD and pressurized liquid extraction
Author(s) -
Wan JianBo,
Li Peng,
Li Shaoping,
Wang Yitao,
Dong Tina TingXia,
Tsim Karl WahKeung
Publication year - 2006
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200600103
Subject(s) - chromatography detector , chromatography , panax notoginseng , repeatability , chemistry , high performance liquid chromatography , extraction (chemistry) , elution , gradient elution , triterpene , medicine , alternative medicine , pathology
A new HPLC coupled with evaporative light scattering detection (ELSD) method was developed for simultaneous determination of 11 major triterpene saponins, namely notoginsenoside R1 ( 1 ), ginsenosides Rg1 ( 2 ), Re ( 3 ), Rf ( 4 ), Rb1 ( 5 ), Rg2 ( 6 ), Rc ( 7 ), Rb2 ( 8 ), Rb3 ( 9 ), Rd ( 10 ), and Rg3 ( 11 ) in Panax notoginseng , a commonly used traditional Chinese medicine (TCM). Pressurized liquid extraction (PLE) was employed for sample preparation, and the analysis was achieved using a Zorbax ODS C 18 column eluted with gradient water‐ACN in 60 min. The drift tube temperature of ELSD was set at 60°C, and nitrogen flowrate was at 1.4 L/min. The method provided good repeatability and sensitivity for quantification of 11 saponins with overall precision (including intra‐ and interday) and LOD of less than 2.9% (RSD) and 98 ng, respectively. The validated method was successfully applied to quantify 11 saponins in 28 samples of P. notoginseng collected in different places, which is helpful to control the quality of P. notoginseng and its related products.