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Fast chiral separation by ligand‐exchange HPLC using a dynamically coated monolithic column
Author(s) -
Schmid Martin G.,
Schreiner Karin,
Reisinger Daniela,
Gübitz Gerald
Publication year - 2006
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200600102
Subject(s) - monolithic hplc column , chromatography , high performance liquid chromatography , column (typography) , chemistry , chiral column chromatography , separation (statistics) , ligand (biochemistry) , chiral stationary phase , separation method , computer science , biochemistry , receptor , telecommunications , frame (networking) , machine learning
The preparation and application of dynamically coated ligand‐exchange chromatography phases for enantioseparation is described. The phases were prepared by pumping a solution of N ‐decyl‐ L ‐4‐hydroxyproline, N ‐hexadecyl‐ L ‐4‐hydroxyproline, or N ‐2‐hydroxydodecyl‐ L ‐4‐hydroxyproline through a commercially available monolithic RP‐18 column. These coatings are stable against desorption for months at ambient temperature when aqueous mobile phases are used. The columns were applied to the chiral separation of amino acids, glycyl dipeptides and diastereomeric dipeptides, and tripeptides. The chiral selector can be removed or changed easily by washing the column with ACN or methanol. Ultrafast separations in the range of seconds were achieved using high flow rates.