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Determination of apomorphine in canine plasma by liquid chromatography–electrospray ionization mass spectrometry and its application to a pharmacokinetic study
Author(s) -
Yang Bei,
Yu Yunqiu,
Cai Lei,
Deng Chunhui,
Duan Gengli
Publication year - 2006
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200600085
Subject(s) - chromatography , chemistry , pharmacokinetics , apomorphine , mass spectrometry , electrospray ionization , analyte , extraction (chemistry) , electrospray , selected reaction monitoring , tandem mass spectrometry , agonist , pharmacology , medicine , biochemistry , receptor
An LC‐ESI‐MS method was developed and validated for the assay of apomorphine in canine plasma using one‐step liquid–liquid extraction. The analytes were separated on a Phenomenex Gemini C 18 (150 mm×2.0 mm id 3 μm) column and determined by MS in the positive ion mode. The linear range was 0.4–40 ng/mL with an LOD of 0.2 ng/mL for apomorphine in plasma. The intraday and interday precision and accuracy of quality control samples were < 5.9% RSD and < 7.5% bias for apomorphine. Extraction recoveries were > 80%. The validated method was successfully applied to analyze canine plasma samples in a pharmacokinetic study of apomorphine in dogs and detailed pharmacokinetic parameters were calculated.