Premium
Separation of parabens in capillary electrochromatography using poly(styrene‐divinylbenzene‐methacrylic acid) monolithic column
Author(s) -
Huang HsiYa,
Huang IYun,
Lin Heng Yi
Publication year - 2006
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200600071
Subject(s) - capillary electrochromatography , divinylbenzene , monolithic hplc column , methacrylic acid , electrochromatography , styrene , polymerization , chromatography , chemistry , monomer , methacrylate , solvent , electrolyte , copolymer , high performance liquid chromatography , polymer , capillary electrophoresis , organic chemistry , electrode
In this study, a series of poly(styrene‐divinylbenzene‐methacrylic acid) monolithic capillaries was used as the separation column of CEC for the analyses of parabens in commercial pharmaceutical and cosmetic products. The results showed that the chromatographic characteristics of these analytes were strongly affected by the preparation condition of the monolithic column including monomer content, porogenic solvent composition, and polymerization time. Baseline separations were markedly sped up by lowering the polymerization time without any obvious loss of resolution. Furthermore, mobile‐phase composition (pH, ACN, and electrolyte concentration) was also able to effectively improve the separation behavior. Similar to the influence of lowering the polymerization time, retention times for all analytes were significantly shortened in the CEC method by decreasing the electrolyte concentration in the mobile phase.