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Fast HPLC method for the determination of glimepiride, glibenclamide, and related substances using monolithic column and flow program
Author(s) -
El Deeb Sami,
Schepers Udo,
Wätzig Hermann
Publication year - 2006
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200600056
Subject(s) - glibenclamide , glimepiride , chromatography , monolithic hplc column , column (typography) , high performance liquid chromatography , chemistry , flow (mathematics) , computer science , mathematics , biology , telecommunications , geometry , frame (networking) , type 2 diabetes , diabetes mellitus , endocrinology
Abstract This work presents a fast method for the simultaneous separation and determination of glimepiride, glibenclamide, and two related substances by RP LC. The separation was performed on a Chromolith Performance (RP‐18e, 100 mm×4.6 mm) column. As mobile phase, a mixture of phosphate buffer pH 3, 7.4 mM, and ACN (55 : 45 v/v) was used. Column oven temperature was set to 30°C. The total chromatographic run time was 80 s. This was achieved using a flow program from 5 to 9.9 mL/min. Precisions of the interday and the intraday assay for both retention times and peak areas for the four analyzed compounds were less than 1.2%. The method showed good linearity and recovery. The short analysis time makes the method very valuable for quality control and stability testing of drugs and their pharmaceutical preparations.