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A rapid and sensitive LC‐MS/MS method for determination of coenzyme Q 10 in tobacco ( Nicotiana tabacum L.) leaves
Author(s) -
Zu Yuangang,
Zhao Chunjian,
Li Chunying,
Zhang Lin
Publication year - 2006
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200600047
Subject(s) - chromatography , chemistry , detection limit , formic acid , electrospray ionization , extraction (chemistry) , coenzyme q10 , nicotiana tabacum , tandem mass spectrometry , mass spectrometry , sample preparation , acetic acid , biochemistry , gene
A rapid and sensitive liquid chromatography‐tandem mass spectrometry method with multiple reaction monitoring has been proposed for the analysis of coenzyme Q 10 in (CoQ 10 ) tobacco leaves. The method used electrospray ionization with detection in positive ion mode. Sample pretreatment involved ultrasonic extraction of fresh tobacco leaves with anhydrous ethanol for 15 min and followed by extraction of the supernatant with hexane. The separation of CoQ 10 was performed on a Symmetry Shield RP18 column with a mixture of acetonitrile and isopropanol (8 : 7, v/v) containing 0.5% formic acid as mobile phase. Quantification of CoQ 10 was performed by the standard addition method. The limit of detection and limit of quantitation of CoQ 10 were, respectively, 1.2 ng/mL ( S/N = 3) and 4.0 ng/mL ( S/N = 10). The relative standard deviations of peak area were 0.91% and 1.21% for intra‐day and inter‐day, respectively. The recoveries of CoQ 10 ranged from 98.2 to 99.3% and the corresponding RSDs were less than 2.4%. Analysis took 5 min, making the method suitable for rapid determination of CoQ 10 in tobacco leaves. The proposed method has been successfully applied to the analysis of CoQ 10 in the leaves from eight varieties of tobacco.