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Simultaneous determination of aromatic and terpenic constituents of cloves by means of HPLC with diode array detection
Author(s) -
Musenga Alessandro,
Ferranti Anna,
Saracino Maria Addolorata,
Fanali Salvatore,
Raggi Maria Augusta
Publication year - 2006
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200600023
Subject(s) - eugenol , chromatography , chemistry , chromatography detector , high performance liquid chromatography , triethylamine , analyte , organic chemistry
An HPLC method has been developed for the simultaneous determination of aromatic and terpenic constituents of cloves on a C8 RP column, with the mobile phase consisting of a pH 3.5 phosphate buffer‐triethylamine (30%) and acetonitrile (70%); a flow rate of 1.2 mL/min and a diode‐array detector were used. Complete separation of all analytes (eugenol (EUG), eugenol acetate (AEUG), β‐caryophyllene, α‐humulene and caryophyllene oxide) was achieved within 7 min. Good linearity was found in the range 0.125–40.0 μg/mL for EUG and AEUG and in the range 0.250–20.0 μg/mL for the terpenic compounds. After validation, the method was successfully applied to the analysis of clove oil and clove extract samples. The results obtained indicate good accuracy (recovery percentage mean value corresponding to 99.9%) and satisfactory precision.