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Preparation and evaluation of a novel chiral stationary phase based on covalently bonded chitosan for ligand‐exchange chromatography
Author(s) -
Liu Yueqi,
Zou Hanfa,
Haginaka Jun
Publication year - 2006
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200600015
Subject(s) - enantiomer , chemistry , covalent bond , chitosan , ligand (biochemistry) , copper , chiral derivatizing agent , chiral column chromatography , ion exchange , chromatography , chiral ligand , column chromatography , resolution (logic) , methanol , ion chromatography , high performance liquid chromatography , phase (matter) , chiral stationary phase , amino acid , carboxylic acid , enantioselective synthesis , organic chemistry , ion , catalysis , biochemistry , receptor , artificial intelligence , computer science
Abstract A novel chiral stationary phase based on chitosan covalently bonded onto silica gels has been prepared and used for the separation of various α‐amino acid enantiomers as well as α‐hydroxycarboxylic acid enantiomers by chiral ligand‐exchange chromatography with copper(II) as a complexing ion. The methanol content and copper(II) ion concentration in the eluent affected retentivity and enantioselectivity. Furthermore, a plausible chiral recognition mechanism for resolution of α‐amino acids was proposed.