Premium
Supercritical CO 2 extraction of emodin and physcion from Polygonum cuspidatum and subsequent isolation by semipreparative chromatography
Author(s) -
Lu HongMei,
Ni WangDong,
Liang YiZeng,
Man RuiLin
Publication year - 2006
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200600012
Subject(s) - emodin , chromatography , extraction (chemistry) , anthraquinone , anthraquinones , high performance liquid chromatography , chemistry , supercritical fluid , supercritical fluid extraction , methanol , organic chemistry , botany , biology
Emodin and physcion are abundant anthraquinone compounds found in the traditional Chinese medicinal herb Polygonum cuspidatum Sied. et Zucc. In this paper, emodin and physcion were successfully extracted with supercritical CO 2 plus ethanol modifier after the extraction conditions were optimized with uniform design‐sequential optimization. Results showed that the ethanol modifier concentration was the main factor for the effective extraction of the emodin. The optimal extraction condition was obtained: 20 MPa, 30°C, and 95% ethanol, at which the yields of emodin and physcion were 0.616 and 0.178 g/100 g, respectively. The yield obtained by supercritical fluid extraction (SFE) was a little lower than that obtained by sonication extraction (SE). The crude extract obtained by SFE was further isolated and purified by semipreparative chromatography with the mobile phase composed of methanol‐water (90 : 1, v/v). Emodin and physcion were obtained with purity 98.6 and 99.1%, respectively, when determined by HPLC, and identification was performed by retention time and UV spectra of the standards. The result suggested that SFE is an alternative and promising method for extraction of the two compounds from P. cuspidatum owing to its environment‐friendly properties and fewer coextracts.