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Retention behaviour of beta‐blockers in HPLC using a monolithic column
Author(s) -
Hashem Hisham,
Jira Thomas
Publication year - 2006
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200600001
Subject(s) - chromatography , chemistry , high performance liquid chromatography , volumetric flow rate , monolithic hplc column , beta (programming language) , buffer (optical fiber) , salt (chemistry) , phase (matter) , reversed phase chromatography , analytical chemistry (journal) , organic chemistry , telecommunications , physics , quantum mechanics , computer science , programming language
The chromatographic behaviour of a new HPLC‐stationary phase (Chromolith® RP‐18e) is described for separation of beta‐blockers. The effects of the different chromatographic conditions (buffer system, pH value, content of organic modifier, injection volume and flow rate) on the separation behaviour were studied. At higher flow rates the peaks seemed to be more symmetrical than at lower flow rates. The use of buffer or salt in the mobile phase for this separation is found to be very essential and the counter‐anion type of this buffer or salt significantly affected the retention behaviour of beta‐blockers while the cation type did not play the same important role.