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2,6‐Dimethyl‐4‐quinolinecarboxylic acid N ‐hydroxysuccinimide ester: A fluorogenic hydrophilic derivatizing reagent for liquid chromatographic analysis of aliphatic amines
Author(s) -
Wang Hong,
Liu Xin,
Zhang Niu,
Cao LiWei,
Zhang HuaShan
Publication year - 2006
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200500508
Subject(s) - chemistry , derivatization , reagent , chromatography , amine gas treating , hydrolysis , high performance liquid chromatography , fluorescence , carboxylic acid , aqueous solution , hydrolysate , detection limit , organic chemistry , physics , quantum mechanics
The use of a fluorogenic, hydrophilic, and amine‐reactive reagent, 2,6‐dimethyl‐4‐quinolinecarboxylic acid N ‐hydroxysuccinimide ester (DMQC‐OSu) has been investigated in the procolumn derivatization for the LC separation of aliphatic amines. In pH 8.0 aqueous medium, DMQC‐OSu reacted with amines at 50°C within 20 min to form highly fluorescent carboxamides and the excess reagent hydrolyzed to the corresponding carboxylic acid. The separation of representative amine derivatives with DMQC‐OSu has been performed using a C 18 column with the fluorescence detection at 326/409 nm. The detection limits reached nanomolar level. The proposed method has been applied to the analysis of real samples with recoveries of 94–108%. Compared with other succinimidyl esters used in the derivatization of amino compounds, DMQC‐OSu and its hydrolysate had negligible fluorescence (ϕ fl = 0.09 and 0.02, respectively), which implied that small peaks appeared in chromatograms and slight interference was introduced to the determination.