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Evaluation of the detection of biomolecules in capillary electrophoresis by contactless conductivity measurement
Author(s) -
AbadVillar Eva M.,
Kubán� Pavel,
Hauser Peter C.
Publication year - 2006
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200500495
Subject(s) - chemistry , capillary electrophoresis , biomolecule , chromatography , amino acid , conductivity , detection limit , analytical chemistry (journal) , peptide , capillary action , electrophoresis , materials science , biochemistry , composite material
Abstract The sensitivity of contactless conductivity detection to amino acids, peptides and proteins in CE was studied for BGE solutions of different pH values. The LOD and analytical characteristics were compared for acidic and basic conditions and better results were in most cases found for buffers of low pH values. Linear dynamic ranges varied between two orders of magnitude for amino acids and peptides and three orders of magnitude for larger proteins. The concentration detection limits were found to be between 1.2 and 7.5 μM for the amino acids tested and for the larger molecules they varied between 2.6 μM for leucine enkephalin and 0.2 μM for HSA when using a buffer at pH 2.1.