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A 150 μm id packed column for the separation of soybean proteins by elution gradient μ‐HPLC: Simultaneous separation of soybean proteins from cereal and milk proteins
Author(s) -
Marina Maria Luisa,
Thorogate Richard,
Smith Norman William
Publication year - 2006
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200500493
Subject(s) - chromatography , chemistry , soybean proteins , resolution (logic) , acetic acid , high performance liquid chromatography , elution , reagent , soy protein , food science , biochemistry , organic chemistry , artificial intelligence , computer science
μ‐HPLC has previously been used to increase the resolution and sensitivity of protein separations but never for the analysis of soybean proteins. In this work, soybean proteins were, for the first time, separated using a capillary column with an internal diameter of 150 μm packed with a Genesis C18 stationary phase (4 μm, 300 Å) and UV detection. TFA and acetic acid were investigated as ion‐pairing reagents in order to optimise water‐ACN gradients to achieve this separation. The column showed good selectivity enabling the separation of soybean proteins from other vegetable proteins such as cereal (wheat, rice and corn) and also from milk proteins. The developed method was applied to the detection of soybean proteins in commercial products elaborated with mixtures of vegetable proteins.