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Molecular imprinting of natural flavonoid antioxidants: Application in solid‐phase extraction for the sample pretreatment of natural products prior to HPLC analysis
Author(s) -
Theodoridis Georgios,
Lasáková Martina,
Škeříková Veronika,
Tegou Andromachi,
Giantsiou Nicoletta,
Jandera Pavel
Publication year - 2006
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200500492
Subject(s) - chromatography , solid phase extraction , high performance liquid chromatography , chemistry , flavonoid , sample preparation , molecular imprinting , natural compound , reversed phase chromatography , extraction (chemistry) , organic chemistry , antioxidant , biochemical engineering , selectivity , catalysis , engineering
As shown in the past years, SPE based on molecularly imprinted polymers (MIPs) may provide significant enhancement of selectivity in sample preparation and analyte preconcentration. The objective of this work was the fabrication of MIPs for the specific adsorption of rutin and quercetin. The two flavonoids were used as the template molecules for the preparation of MIP phases in a self‐assembly (noncovalent) approach. The produced MIPs were validated with regard to the imprinting efficiency as media for LC and SPE. The retention behavior of several flavonoid compounds was studied using as stationary phases imprinted, control nonimprinted polymers, and commercial silica‐based materials. MIPs were applied as materials for the selective SPE and preconcentration of the flavonoids from white and red wine, orange juice, and tea. The collected fractions were analyzed by high‐pressure LC. MIP‐SPE facilitated specific analyte isolation and effective sample clean‐up. The results show that molecularly imprinted SPE can be a useful tool for the simple, selective, and cost‐effective pretreatment of samples containing natural antioxidants.