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Separation of triacylglycerols in a complex lipidic matrix by using comprehensive two‐dimensional liquid chromatography coupled with atmospheric pressure chemical ionization mass spectrometric detection
Author(s) -
Dugo Paola,
Kumm Tiina,
Chiofalo Biagina,
Cotroneo Antonella,
Mondello Luigi
Publication year - 2006
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200500476
Subject(s) - chromatography , chemistry , atmospheric pressure chemical ionization , atmospheric pressure , matrix (chemical analysis) , chemical ionization , mass spectrometry , ionization , analytical chemistry (journal) , high performance liquid chromatography , complex matrix , direct electron ionization liquid chromatography–mass spectrometry interface , organic chemistry , ion , oceanography , geology
The present investigation describes the employment of a comprehensive 2‐D HPLC system, based on the combination of a silver ion and an RP column, for the characterization of the triacylglycerol (TAG) fraction of a very complex lipidic sample: donkey milk fat. The TAGs were grouped on the resulting bidimensional contour plot according to their double bond numbers (aligned along vertical bands) and according to their partition numbers (aligned along horizontal bands). Peak assignment was supported by using atmospheric pressure chemical ionization mass spectrometric (APCI‐MS) detection. The combination of the enhanced resolving power of comprehensive multidimensional LC, the formation of ordered 2‐D patterns, and APCI‐MS detection proved to be an effective tool for the characterization of the complex matrix, enabling the separation and identification of nearly 60 TAGs.