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Preparative isolation and purification of atractylon and atractylenolide III from the Chinese medicinal plant Atractylodes macrocephala by high‐speed counter‐current chromatography
Author(s) -
Zhao Chunxia,
He Chaohong
Publication year - 2006
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200500464
Subject(s) - chromatography , countercurrent chromatography , chemistry , elution , radix (gastropod) , ethyl acetate , high performance liquid chromatography , botany , biology
The medicinal plant Atractylodes macrocephala (Baizhu in Chinese) has been widely used in traditional Chinese medicine for energy and stomach complaints, treatment of dyspepsia and anorexia, anti‐inflammation, anticancer and for increasing assimilation. A high‐speed counter‐current chromatography (HSCCC) method was developed for the preparative separation and purification of two main bioactive components, namely, atractylon and atractylenolide III from A. macrocephala by using light petroleum (60–90°C)–ethyl acetate–ethanol–water (4 : 1 : 4 : 1 v/v) as the two‐phase solvent system in dual‐mode elution. Compared with the separation using the normal‐mode elution, the dual‐mode HSCCC can be achieved with shorter elution time. Atractylenolide III (32.1 mg) at 99.0% purity and 319.6 mg atractylon at 97.8% purity could be obtained from 1000 mg crude sample in a single run. The recoveries of atractylenolide III and atractylon were 95.4 and 92.6%, respectively.