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Development and validation of HPLC methods for the analysis of phenethylamine and indoloquinazoline alkaloids in Evodia species
Author(s) -
Pellati Federica,
Benvenuti Stefania,
Yoshizaki Fumihiko,
Melegari Michele
Publication year - 2006
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200500460
Subject(s) - evodiamine , rutaecarpine , phenethylamine , chemistry , chromatography , high performance liquid chromatography , alkaloid , palmatine , rutaceae , jatrorrhizine , officinalis , traditional medicine , stereochemistry , botany , biology , medicine
The aim of this study was to evaluate the chromatographic performance of a PEG stationary phase, in comparison with those of C 18 columns, for the HPLC analysis of phenethylamine ((±)‐synephrine) and indoloquinazoline (rutaecarpine and evodiamine) alkaloids in methanolic extracts of fruits of Evodia rutaecarpa (Juss.) Benth. and E. rutaecarpa (Juss.) Benth. var. officinalis (Dode) Huang ( i. e. , E. officinalis Dode) (Rutaceae family). The method was validated and showed good linearity, precision, accuracy, sensitivity, and specificity. The highest content of both phenethylamine and indoloquinazoline alkaloids was found in methanolic fruit extracts of E. rutaecarpa , and it was closely related to the degree of maturity. E. officinalis fruits displayed low amounts of both types of alkaloids. Furthermore, an enantioselective HPLC method for the enantioseparation of (±)‐synephrine from Evodia fruits was applied, by using a protein‐based chiral stationary phase with cellobiohydrolase (CBH) as the chiral selector (Chiral‐CBH). Isolation of synephrine from Evodia aqueous fruit extracts was carried out by strong cation‐exchange SPE. The results of the application of the method to the analysis of Evodia samples showed that (–)‐synephrine was the main component while (+)‐synephrine was present in low concentration.