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Direct determination of ciprofloxacin by mass spectrometry after a two‐step solid‐phase extraction using a molecularly imprinted polymer
Author(s) -
Caro Ester,
Marcé Rosa M.,
Cormack Peter A. G.,
Sherrington David C.,
Borrull Francesc
Publication year - 2006
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200500439
Subject(s) - molecularly imprinted polymer , chromatography , solid phase extraction , cartridge , chemistry , sorbent , extraction (chemistry) , mass spectrometry , sample preparation , molecular imprinting , matrix (chemical analysis) , adsorption , materials science , selectivity , organic chemistry , metallurgy , catalysis
A molecularly imprinted polymer (MIP) has been prepared for the first time with ciprofloxacin (CIPRO) as the template molecule, via a noncovalent synthetic procedure. Prior to its use as a sorbent in SPE, the MIP was evaluated chromatographically to confirm that it was indeed molecularly imprinted. The MIP was then used to extract CIPRO selectively from urine samples by means of a two‐step SPE procedure in which a commercial Oasis cartridge and a molecularly imprinted SPE cartridge were combined in series. This approach allowed the matrix compounds present in the samples to be removed effectively. The urine extracts obtained after this two‐step SPE procedure was applied were relatively clean compared to the original samples, and this made it possible to inject directly the extracts into a mass spectrometer and thus quantify CIPRO in urine samples at low levels and reduce the time of analysis.