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Solid‐phase extraction of 4(5)‐methylimidazole (4MeI) and 2‐acetyl‐4(5)‐(1,2,3,4‐tetrahydroxybutyl)‐imidazole (THI) from foods and beverages with subsequent liquid chromatographic‐electrospray mass spectrometric quantification
Author(s) -
Klejdus Bořivoj,
Moravcová Jitka,
Lojková Lea,
Vacek Jan,
Kubáň Vlastimil
Publication year - 2006
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200500421
Subject(s) - chromatography , chemistry , solid phase extraction , calibration curve , electrospray , extraction (chemistry) , analyte , mass spectrometry , imidazole , high performance liquid chromatography , sample preparation , detection limit , stereochemistry
A method for the simultaneous determination of 4(5)‐methylimidazole (4MeI) and 2‐acetyl‐4(5)‐(1,2,3,4‐tetrahydroxybutyl)‐imidazole (THI) was developed using SPE and HPLC/MS. Solid‐phase extraction using SCX Disc cartridges was used for isolation of the analytes from liquid samples. The lower LOQ was 0.1 ng/mL for 4MeI and 0.2 ng/mL for THI. The linearity of the calibration curves was satisfactory as indicated by correlation coefficients > 0.999. The CV for the intra‐ and inter‐day precision was < 5% ( n = 6); the accuracy was in the range 98–103%. The recovery was ⪈ 97 and ⪈ 98% for THI and 4MeI, respectively. The method was used to determine THI and 4MeI in beverages, coffee, caramel colours and other samples.