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Field‐amplified on‐line sample stacking for determination of carnosine‐related peptides by capillary electrophoresis
Author(s) -
Huang Ying,
Shi Yanfang,
Duan Jianping,
Chen Guonan
Publication year - 2006
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200500402
Subject(s) - capillary electrophoresis , stacking , chromatography , chemistry , field (mathematics) , sample (material) , carnosine , sample preparation , analytical chemistry (journal) , biochemistry , organic chemistry , mathematics , pure mathematics
An on‐line sample stacking method, namely field‐amplified sample injection, has been developed for the separation and determination of carnosine, anserine, and homocarnosine by capillary electrophoresis. Using electrokinetic injection, about 130‐ to 160‐fold improvement of sensitivity was achieved without loss of separation efficiency when compared to conventional sample injection. For conventional injection, the samples were dissolved in running buffer and then hydrodynamically injected for 10 s (3.45 kPa). Various parameters affecting separation and sample stacking were optimized. Under optimum conditions, linear responses were obtained over two orders of magnitude and the detection limits (defined as S/N = 3) of carnosine, anserine, and homocarnosine were 1.5×10 –8 to 1.6×10 –8 mol/L.