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High‐performance liquid chromatographic enantioseparations on capillary columns containing crosslinked polysaccharide phenylcarbamate derivatives attached to monolithic silica
Author(s) -
Chankvetadze Bezhan,
Kubota Takateru,
Ikai Tomoyuki,
Yamamoto Chiyo,
Kamigaito Masami,
Tanaka Nobuo,
Nakanishi Kazuki,
Okamoto Yoshio
Publication year - 2006
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200500388
Subject(s) - chemistry , capillary action , chromatography , capillary electrophoresis , covalent bond , polysaccharide , cellulose , capillary electrochromatography , enantiomer , organic chemistry , materials science , composite material
Monolithic capillary columns containing native silica gel were covalently modified with 3,5‐disubstituted phenylcarbamate derivatives of cellulose and amylose and applied for enantioseparations in capillary LC. The method previously used for covalent immobilization of polysaccharide phenylcarbamate derivatives onto the surface of microparticulate silica gel was successfully adapted for in situ modification of monolithic fused‐silica capillary columns. The effects of the nature of polysaccharide and the substituents, as well as of multiple covalent immobilization of polysaccharide derivative on chromatographic performance of capillary columns were studied. The capillary columns obtained using this technique are stable in all solvents commonly used in LC and exhibit promising enantiomer resolving ability.