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Determination of paralytic shellfish toxins in dinoflagellate Alexandrium tamarense by using isotachophoresis/capillary electrophoresis
Author(s) -
Wu Youyi,
Ho Alvin Yam Tat,
Qian PeiYuan,
Leung Kelvin SzeYin,
Cai Zongwei,
Lin JinMing
Publication year - 2006
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200500386
Subject(s) - alexandrium tamarense , saxitoxin , chromatography , capillary electrophoresis , isotachophoresis , shellfish , chemistry , detection limit , paralytic shellfish poisoning , toxin , biology , algal bloom , fishery , fish <actinopterygii> , aquatic animal , biochemistry , organic chemistry , phytoplankton , electrode , nutrient , electrolyte
Baseline separation of seven paralytic shellfish toxins (PSTs), namely decarbamoylsaxitoxin (dcSTX), saxitoxin (STX), neosaxitoxin (NEO), gonyautoxin‐2 (GTX‐2), gonyautoxin‐3 (GTX‐3), gonyautoxin‐1 (GTX‐1), and gonyautoxin‐4 (GTX‐4), was achieved by using capillary ITP (CITP)/CE with UV detection. Separation parameters including duration time and voltage in CITP process, separation voltage, and pH and concentration of buffer were optimized. The developed method provided linear responses from 1.3 to 200 μM for the PSTs. The LOD ranged from 0.1 to 0.3 μM. PST extracts from two algal strains of Alexandrium tamarense were analyzed and the toxin concentrations in the samples were quantified with an internal standard method by using NEO as the internal standard. The algal extract of A. tamarense HK9301 contained 332 μM GTX‐2 and 224 μM GTX‐3, while the PSTs were not detected in the extract of A. tamarense CI01.