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Hydrazine determination in sludge samples by high‐performance liquid chromatography
Author(s) -
Elias Gracy,
Bauer William F.
Publication year - 2006
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200500380
Subject(s) - hydrazine (antidepressant) , chromatography , high performance liquid chromatography , detection limit , chemistry , hydrazone , methanol , benzaldehyde , organic chemistry , catalysis
A relatively simple and cost‐effective method utilizing HPLC with UV detection was developed to detect and quantify hydrazine in sludge samples. The method was developed primarily for sludge samples, but it can also be applicable to soil and other environmental samples. The hydrazines in the matrices were derivatized to hydrazones with benzaldehyde. The hydrazones were separated using HPLC with an RP C‐18 column in an isocratic mode with methanol–water (95:5 v/v) and detected with UV detection at 313 nm. The detection limit (25 μL injection) for the method is 0.02 μg/mL of hydrazine.