Premium
Separation and determination of 2,4‐D, dicamba and 2,4,5‐T in tobacco by nonaqueous capillary electrophoresis
Author(s) -
Liu Hongxia,
Song Juanmei,
Han Po,
Li Yongbo,
Zhang Shusheng,
Liu Huimin,
Wu Yangjie
Publication year - 2006
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200500373
Subject(s) - capillary electrophoresis , chromatography , dicamba , chemistry , capillary action , analytical chemistry (journal) , materials science , biology , weed control , agronomy , composite material
A practical method for residue analysis of 2,4‐D, dicamba and 2,4,5‐T in baked tobacco leaves has been developed using nonaqueous CE (NACE). The herbicide residues of 2,4‐D, dicamba and 2,4,5‐T in tobaccos were extracted by ultrasonication with ethyl acetate, followed by a cleanup procedure with gel permeation chromatography. The separation of 2,4‐D, dicamba and 2,4,5‐T by NACE was optimized based on orthogonal experiment design with four factors at three levels. The optimal NACE condition was established with the running buffer of 40.0 mmol/L ammonium acetate in 90% CH 3 CN (apparent pH 10.2), and the applied voltage of –25 kV over a capillary of 50 μm id×46 cm (37.5 cm to the detector window), which gave a baseline separation of 2,4‐D, dicamba and 2,4,5‐T within 15 min. The LOD were ca. 0.4–0.6 μg/mL for the three herbicides, whereas the overall recovery ranged from 80.8 to 84.1%. The proposed method has been successfully applied to measure 300 real tobacco samples, and the residue profiles of the three herbicides in tobacco samples were obtained and evaluated.