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A comparative study of upright counter‐current chromatography and high‐performance liquid chromatography for preparative isolation and purification of phenolic compounds from Magnoliae officinalis
Author(s) -
Lu Yanbin,
Sun Cuirong,
Pan Yuanjiang
Publication year - 2006
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200500363
Subject(s) - magnolol , honokiol , chromatography , chemistry , high performance liquid chromatography , countercurrent chromatography , magnolia officinalis , ethyl acetate , salidroside , solvent , officinalis , extraction (chemistry) , organic chemistry , medicine , linguistics , philosophy , alternative medicine , traditional chinese medicine , pathology
A comparative study of preparative isolation and purification of the phenolic compounds magnolol and honokiol from the Chinese medicinal plant Magnoliae officinalis by upright counter‐current chromatography (CCC) and semi‐preparative HPLC is presented. The comparison reveals that with a two‐phase solvent system composed of light petroleum (bp 60–90°C)–ethyl acetate–tetrachloromethane–methanol–water (1:1:8:6:1, v/v), 1250 mg of honokiol and 520 mg of magnolol, with a purity of 98.7 and 99.5%, respectively, were obtained from 2.0 g of a crude sample of Magnoliae officinalis in a single CCC separation. In contrast, semi‐preparative HPLC allowed isolation and purification of these two phenolic compounds with significantly lower productivity and higher solvent consumption. Structures of the purified compounds were identified by 1 H and 13 C NMR.