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A new capillary zone electrophoresis method for the analysis of Ginkgo biloba extracts and Ginkgo biloba pharmaceutical products
Author(s) -
Shaban Elosta,
Dagmar Gajdošová,
Josef Havel
Publication year - 2006
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200500361
Subject(s) - ginkgo biloba , chromatography , capillary electrophoresis , chemistry , ginkgoales , pharmacognosy , botany , biochemistry , biological activity , biology , in vitro
Ginkgo biloba , traditional Chinese medicine is now generally accepted. Separation and determination of active components in G. biloba is important for the product quality control. Therefore, the development of an effective and reliable separation method is important. In this work, a new capillary electrophoretic (CZE) method for separation of the G. biloba leaf extracts components was developed and optimized by the use of experimental design and artificial neural network (ANN). Under best separation conditions, in γ‐CD‐modified buffer, the separation was reached within 10 min (36 mM borate BGE, pH 9.2, 1 mM γ‐CD), while the hydrodynamic mode for sample injection (2 s) and UV detection at 270 nm were applied. The method developed was validated and applied for analysis of various extracts and G. biloba products.