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Microemulsion electrokinetic chromatography for separation and analysis of curcuminoids in turmeric samples
Author(s) -
Nhujak Thumnoon,
Saisuwan Warangkana,
Srisaart Monpichar,
Petsom Amorn
Publication year - 2006
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200500333
Subject(s) - microemulsion , chromatography , chemistry , resolution (logic) , extraction (chemistry) , electrokinetic phenomena , pulmonary surfactant , dilution , sample preparation , analytical chemistry (journal) , biochemistry , physics , artificial intelligence , computer science , thermodynamics
Microemulsion EKC (MEEKC) was developed for quantitative analysis of curcuminoids, such as curcumin (C), demethoxycurcumin (D), and bis‐demethoxycurcumin (B). MEEKC separation of curcuminoids was optimized, and a change in resolution was explained using a modified equation for resolution in MEEKC without electroosmosis. The suitable MEEKC conditions for separation of curcuminoids were obtained to be the microemulsion buffer containing 50 mM phosphate buffer at pH 2.5, 1.1% v/v n ‐octane as oil droplets, 180 mM SDS as surfactant, 890 mM 1‐butanol as cosurfactant, and 25% v/v 2‐propanol as organic cosolvent; applied voltage of –15 kV; and separation temperature 25°C. Achieved baseline resolution of C : D and D : B was obtained with R s ˜2.4 and analysis time within 18 min. In addition, high accuracy and precision of the method were obtained. This MEEKC method was used for quantitative determination of individual curcuminoids in medicinal turmeric capsules and powdered turmeric used as coloring additive in food, with simple sample preparation such as solvent extraction, dilution, and filtration, and without cleaning up by SPE.