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Application of cyclodextrins as modifiers in electrophoretic separation of metalloporphyrins
Author(s) -
Pyrzyńska Krystyna,
Kilian Krzysztof
Publication year - 2006
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200500330
Subject(s) - chemistry , capillary electrophoresis , cyclodextrin , boron , metal , resolution (logic) , chromatography , electrophoresis , buffer (optical fiber) , analytical chemistry (journal) , organic chemistry , artificial intelligence , computer science , telecommunications
Several metallocomplexes of tetrakis ‐carboxyphenylporphyrin (TCPP) were separated on fused‐silica capillary using CZE with UV‐VIS detection. Metalloporphyrins of Co(II), Cu(II), Mn(II), Ni(II), and Zn(II) were formed directly in TCPP solution with addition of Cd(II) to increase the formation reaction rate. The composition of BGE, its concentration, and pH were optimized to ensure the stability of complexes and proper resolution. In particular, the problem of signals' shape was investigated and discussed. The presence of β‐CD in borate buffer significantly improved separation efficiency and signal shapes due to formation of inclusion complexes. Under the best separation conditions (50 mM borate running buffer at pH 9 with addition of 2 mM β‐CD, 30 kV applied voltage) a separation of metal complexes with TCPP was accomplished in 16 min.